chromatography: Ion-Exchange Chromatography

For compounds that can exist as ions, ion-exchange chromatography can be used to separate them from neutral or oppositely charged compounds. The mixture is added to a column packed with a porous, insoluble resin which has a negatively charged (anionic) group attached to it and an unattached, positively charged (cationic) counterion. A cation from the mixture will exchange with the positive counterion of the resin and will be retarded while neutral and anionic substances are not affected. Ion-exchange resins with exchangeable anions work in a similar manner.

Sections in this article:

• Introduction
• Electrophoresis
• Thin-Layer and Paper Chromatography
• Ion-Exchange Chromatography
• Gel-Permeation Chromatography
• Liquid Chromatography
• Gas Chromatography
• Column Chromatography

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